Inhibitory effect of short-hairpin RNA expression vector- mediated osteopontin RNA interference on proliferation and invasion of prostate cancer PC-3 cells
单位：[1]Department of Orthopedics,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,Hubei 430030,China华中科技大学同济医学院附属同济医院外科学系骨科
Background and Objective: Osteopontin (OPN) regulates cell migration and Invasion in a variety of cancers and induces the activation of matrix metalloproteinase (MMP)-2 and MMP-9. This study was to investigate the role of OPN in the proliferation and Invasion of human prostate cancer PC-3 cells and the possible functions of IkB kinase (IKK) in nuclear factor kappa B (NF-κB)-mediated signaling pathways. Methods: OPN short-hairpin RNA (shRNA) recombinant plasmlds were transfected into PC-3 cells and different concentrations of IKK inhibitors were used to inhibit the activities of IKKα and IKKβ. The mRNA and protein expression levers of OPN, MMP-2, and MMP-9 were detected by real-time polymerase chain reaction (PCR) and Western blot. Cell cycle was detected by flow cytometry, cell proliferation by MTT assay, and cell invasion by Transwell chamber assay. Results: Compared with untransfected cells, the protein levers of OPN, MMP-2, and MMP-9 in OPN shRNA- transfected PC-3 cells were reduced by 55.22%, 51.71%, and 28.35%, respectively, and the abilities of cell migration and invasion were decreased by 45.48% and 51.96%, respectively (P< 0.05). Moreover, the inhibition of IKKβ Inhibited the expressions of MMP-2 and MMP-9. Conclusion: OPN shRNA-mediated OPN gene silencing can inhibit the malignant biological behaviors of PC-3 cells. IKKβ may play a crucial role in the OPN-induced activation of MMP-2 and MMP-9 via NF- κB-mediated IκB/IKKβ pathways.
语种：
外文
第一作者：
第一作者单位：[1]Department of Orthopedics,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,Hubei 430030,China
通讯作者：
推荐引用方式(GB/T 7714)：
Liu H,Chen A.-M,Guo F.-J,et al.Inhibitory effect of short-hairpin RNA expression vector- mediated osteopontin RNA interference on proliferation and invasion of prostate cancer PC-3 cells[J].Chinese Journal of Cancer.2010,29(1):doi:10.5732/cjc.009.10517.
APA：
Liu, H,Chen, A.-M,Guo, F.-J,Liao, H,Hu, W.-H&Xie, J.(2010).Inhibitory effect of short-hairpin RNA expression vector- mediated osteopontin RNA interference on proliferation and invasion of prostate cancer PC-3 cells.Chinese Journal of Cancer,29,(1)
MLA：
Liu, H,et al."Inhibitory effect of short-hairpin RNA expression vector- mediated osteopontin RNA interference on proliferation and invasion of prostate cancer PC-3 cells".Chinese Journal of Cancer 29..1(2010)
