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Cloning, characterization and expression analysis of a cold shock domain family member YB-1 in turbot Scophthalmus maximus

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资源类型：
Pubmed体系：
作者：
Wang, X.-L[a] * ; Zhang, Y.-X[b] ; Yang, C.-G[c] ; Zhang, B[b] ; Chen, S.-L[b] ;
单位： [a]Translational Center for Stem Cell Research, Tongji Hospital, Stem Cell Research Center, Tongji University School of Medicine, Shanghai 200065, China [b]Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Key Lab For Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Qingdao 266071, China [c]Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China
出处：
DOI： 10.1016/j.fsi.2012.09.001
ISSN：

关键词： CDNA Cloning Expression Turbot YP-1

摘要：
The Y-box proteins are a family of highly conserved nucleic acid binding proteins. In this report we have identified a new member, YB-1 from turbot (Scophthalmus maximus) spleen cDNA library. The full-length cDNA sequence of turbot YB-1 was obtained and then the expression at transcriptional level was researched by qRT-PCR. In normal organs, the expression of YB-1 was higher in liver, brain, gill and heart, respectively. YB-1 had the highest expression level at gastrula stage during the early stages of embryo development. In the liver, kidney and spleen, the turbot YB-1 expression level was the highest at 72 h after challenge with lymphocystis disease virus (LCDV) and the highest at 12 h after challenge with Vibrio anguillarum (V. anguillarum). Furthermore, the expression of turbot YB-1 also distinctly increased in turbot kidney cells (TK) at 24 h after challenge with V. anguillarum and LCDV. These results indicated that the turbot YB-1 protein may play a signifi{ligature}cant role in the immune response of turbot. © 2012 Elsevier Ltd.

基金：
This work was supported by grants from National Major Basic Research Program (973) of China ( 2010CB126303 ), State 863 High-Technology R&D Project of China ( 2012AA092203 ) and Taishan Scholar Project of Shandong Province.
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2011]版：
大类 | 1 区 农林科学
小类 | 1 区 兽医学 2 区 渔业 2 区 海洋与淡水生物学 3 区 免疫学
最新[2025]版：
大类 | 2 区 农林科学
小类 | 1 区 渔业 1 区 海洋与淡水生物学 1 区 兽医学 3 区 免疫学
第一作者：
第一作者单位： [a]Translational Center for Stem Cell Research, Tongji Hospital, Stem Cell Research Center, Tongji University School of Medicine, Shanghai 200065, China
通讯作者：
推荐引用方式(GB/T 7714)：
Wang X.-L,Zhang Y.-X,Yang C.-G,et al.Cloning, characterization and expression analysis of a cold shock domain family member YB-1 in turbot Scophthalmus maximus[J].Fish & shellfish immunology.2012,33(5):1215-21.doi:10.1016/j.fsi.2012.09.001.
APA：
Wang, X.-L,Zhang, Y.-X,Yang, C.-G,Zhang, B&Chen, S.-L.(2012).Cloning, characterization and expression analysis of a cold shock domain family member YB-1 in turbot Scophthalmus maximus.Fish & shellfish immunology,33,(5)
MLA：
Wang, X.-L,et al."Cloning, characterization and expression analysis of a cold shock domain family member YB-1 in turbot Scophthalmus maximus".Fish & shellfish immunology 33..5(2012):1215-21

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