Molecular characterization of the type I IFN receptor in two woodchuck species and detection of its expression in liver samples from woodchucks infected with woodchuck hepatitis virus (WHV)
Type I interferons (IFN-alpha/beta) serve as the first line of defense against viral infection and share the same type I IFN receptor (IFNAR) complex, which is composed of IFNAR1 and -2. The Eastern woodchuck (Marmota monax) and Chinese woodchuck (Marmota himalayana) are suitable for studying hepatitis B virus (HBV) infection. Here, the complete or partial sequences of the IFNARs of both species were obtained and analyzed. Small interference RNAs targeting wIFNAR1 and -2 specifically down-regulated the expression of wIFNAR1 and -2 and the IFN-stimulated gene MxA in a woodchuck cell line, respectively. IFNAR2 was significantly up-regulated in primary woodchuck hepatocytes stimulated with IFN-alpha alpha or -gamma. The expression of woodchuck IFNAR1 and -2 was decreased in woodchucks chronically infected with woodchuck hepatitis virus (WHV). These results are essential for studying type I IFN-related innate immunity and therapy in hepadnaviral infection in the woodchuck model. (C) 2012 Elsevier Ltd. All rights reserved.
基金：
National Major Science and Technology Project for Infectious Diseases of China [2008ZX10002-011]; State Major Basic Research Program of China [2005CB522901, 200703512804, 2009CB522506]; National Natural Science Foundation of China [30271170, 8110 1248]; International Science & Technology Cooperation Program of China [2011DFA31030]; Deutsche Forschungsgemeinschaft [Transregio TRR60]
第一作者单位：[1]Huazhong Univ Sci & Technol,Tongji Hosp,Tongji Med Coll,Div Clin Immunol,Wuhan 430074,Peoples R China
通讯作者：
通讯机构：[1]Huazhong Univ Sci & Technol,Tongji Hosp,Tongji Med Coll,Div Clin Immunol,Wuhan 430074,Peoples R China[*1]Huazhong Univ Sci & Technol,Tongji Hosp,Tongji Med Coll,Div Clin Immunol,Jiefang Ave 1095,Wuhan 430074,Peoples R China
推荐引用方式(GB/T 7714)：
Fan Hebin,Zhu Zhenni,Wang Yang,et al.Molecular characterization of the type I IFN receptor in two woodchuck species and detection of its expression in liver samples from woodchucks infected with woodchuck hepatitis virus (WHV)[J].CYTOKINE.2012,60(1):179-185.doi:10.1016/j.cyto.2012.05.013.
APA：
Fan, Hebin,Zhu, Zhenni,Wang, Yang,Zhang, Xiaoyong,Lu, Yinping...&Yang, Dongliang.(2012).Molecular characterization of the type I IFN receptor in two woodchuck species and detection of its expression in liver samples from woodchucks infected with woodchuck hepatitis virus (WHV).CYTOKINE,60,(1)
MLA：
Fan, Hebin,et al."Molecular characterization of the type I IFN receptor in two woodchuck species and detection of its expression in liver samples from woodchucks infected with woodchuck hepatitis virus (WHV)".CYTOKINE 60..1(2012):179-185
