CD4 T cells play an important role in the immune response against hepatitis B virus (HBV) infection. Woodchucks represent an excellent animal model to study HBV infection. In this study, we characterized the cDNA sequence of woodchuck CD4 (wCD4). The deduced wCD4 protein has four extracellular immunoglobulin-like domains comparable to the other mammalian CD4 molecules. The important extracellular cysteine residues and the intracellular tyrosine protein kinase-binding site of wCD4 are also conserved. The deduced wCD4 protein shows 53-63% identity with the counterparts of other mammalians. Phylogenetic analysis indicates that wCD4 is closely related with the counterparts of primates. Two polyclonal antibodies (pAbs) and four monoclonal Abs (mAbs) against wCD4 were produced. Two pAbs and one rnAbs (G2) were found to effectively suppress ConA induced proliferation in vitro. Anti-wCD4 mAb G2 depleted 60% of CD4 cells from healthy woodchucks, while the remaining CD4 cells responded well to ConA stimulation. This work provides a basis for studying CD4 T cell mediated immune responses against HBV infection in the woodchuck model. (C) 2013 Elsevier Ltd. All rights reserved.
基金：
National Major Science and Technology Project for Infectious Diseases of China [2008ZX10002011, 2012ZX10004503]; National Natural Science Foundation of China [81101248]; International Science & Technology Cooperation Program of China [2011DFA31030]; Deutsche Forschungsgemeinschaft DFG [Transregio TRR60]
第一作者单位：[1]Huazhong Univ Sci & Technol, Union Hosp Tongji Med Coll, Dept Infect Dis, Wuhan 430022, Peoples R China[2]Huazhong Univ Sci & Technol, Tonidi Med Coll, Dept Microbiol, Wuhan 430022, Peoples R China
通讯作者：
通讯机构：[*1]Huazhong Univ Sci & Technol, Union Hosp Tongji Med Coll, Dept Infect Dis, Jiefang Ave 1277, Wuhan 430022, Peoples R China
推荐引用方式(GB/T 7714)：
Yang Yinke,Zhang Xiaoyong,Zhang Chunyan,et al.Molecular characterization of woodchuck CD4 (wCD4) and production of a depletion monoclonal antibody against wCD4[J].MOLECULAR IMMUNOLOGY.2013,56(1-2):64-71.doi:10.1016/j.molimm.2013.04.006.
APA：
Yang, Yinke,Zhang, Xiaoyong,Zhang, Chunyan,Ta, Yuanqing,Fan, Wei...&Wang, Baoju.(2013).Molecular characterization of woodchuck CD4 (wCD4) and production of a depletion monoclonal antibody against wCD4.MOLECULAR IMMUNOLOGY,56,(1-2)
MLA：
Yang, Yinke,et al."Molecular characterization of woodchuck CD4 (wCD4) and production of a depletion monoclonal antibody against wCD4".MOLECULAR IMMUNOLOGY 56..1-2(2013):64-71
