Increasing evidence suggests that FOX01, one critical gene related to the human immune system, probable is closely to the human infection. In the present study we aimed to investigate genetic association of FOX01 with bacteremia in Han Chinese. 188 patients with bacteremia diagnosed with blood culture and 250 healthy blood donors without signs of infection were studied, two tatting SNPs of FOX01 (rs9532571, rs3751436) were selected and genotyped using predesigned TaqMan allelic discrimination assays. The results showed that the allele frequency of rs9532571 and rs3751436 in FOX01 was not associated with an increased risk of bacteremia (P=0.762, OR=1.05, 95% 01 0.77-1.43; P=0.059, OR=1.34, 95% Cl 0.99-1.81 respectively), the genotype distribution of these two SNPs was also not significantly different between bacteremia patients and control groups (P=0.9; P=0.16). Haplotypes in this block were not significantly associated with bacteremia risk. Conclusion: the association between FOX01 genetic polymorphism and bacteremia has not been observed in the study, maybe a larger sample population and more SNPs in the FOX01 need to reveal the role in bacteremia in the future.