单位：[1]Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai, China[2]Department of Pathogen Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China[3]Department of Clinical Laboratory, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China华中科技大学同济医学院附属同济医院检验科[4]CAS Key Laboratory of Special Pathogens and Biosafety, Centre for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei, China[5]College of Life Science, Nankai University, Tianjin 300071, China[6]University of Chinese Academy of Sciences, Beijing, China[7]Department of Public Health, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China华中科技大学同济医学院附属同济医院党政职能科室保健科（公共卫生科）[8]Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
To fully decipher the immunogenicity of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Spike protein, it is essential to assess which part is highly immunogenic in a systematic way. We generate a linear epitope landscape of the Spike protein by analyzing the serum immunoglobulin G (IgG) response of 1,051 coronavirus disease 2019 (COVID-19) patients with a peptide microarray. We reveal two regions rich in linear epitopes, i.e., C-terminal domain (CTD) and a region close to the S20 cleavage site and fusion peptide. Unexpectedly, we find that the receptor binding domain (RBD) lacks linear epitope. Wereveal that the number of responsive peptides is highly variable among patients and correlates with disease severity. Some peptides are moderately associated with severity and clinical outcome. By immunizing mice, we obtain linear-epitope-specific antibodies; however, no significant neutralizing activity against the authentic virus is observed for these antibodies. This landscape will facilitate our understanding of SARS-CoV-2-specific humoral responses and might be useful for vaccine refinement.
基金：
National Key Research and Development Program of China [2016YFA0500600]; Science and Technology Commission of Shanghai MunicipalityScience & Technology Commission of Shanghai Municipality (STCSM) [19441911900]; Interdisciplinary Program of Shanghai Jiao Tong University [YG2020YQ10]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31970130, 31600672, 31670831, 31370813]; China Postdoctoral Science FoundationChina Postdoctoral Science Foundation [2020M680857]
第一作者单位：[1]Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai, China[5]College of Life Science, Nankai University, Tianjin 300071, China
共同第一作者：
通讯作者：
通讯机构：[4]CAS Key Laboratory of Special Pathogens and Biosafety, Centre for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei, China[6]University of Chinese Academy of Sciences, Beijing, China
推荐引用方式(GB/T 7714)：
li yang,ma ming-liang,lei qing,et al.Linear epitope landscape of the SARS-CoV-2 Spike protein constructed from 1,051 COVID-19 patients[J].CELL REPORTS.2021,34(13):doi:10.1016/j.celrep.2021.108915.
APA：
li,yang,ma,ming-liang,lei,qing,wang,feng,hong,wei...&tao,sheng-ce.(2021).Linear epitope landscape of the SARS-CoV-2 Spike protein constructed from 1,051 COVID-19 patients.CELL REPORTS,34,(13)
MLA：
li,yang,et al."Linear epitope landscape of the SARS-CoV-2 Spike protein constructed from 1,051 COVID-19 patients".CELL REPORTS 34..13(2021)
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