单位：[1]Department of Urology,Tongji Hospital,Huazhong University of Science and Technology,Wuhan,Hubei 430030,China华中科技大学同济医学院附属同济医院外科学系泌尿外科
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摘要：
Objective: To analyze the biological properties of human corpus cavernosum smooth muscle cells (hCCMCs) under three-dimensional culture system. Methods: hCCMCs were isolated in vitro, and purified by gradient velocity sedimentation. hCCMCs were identified by the expression of α-smooth muscle actin (α-SMA). On the basis of adherent monolayer culture, hCCMCs were seeded into collagen gel for three-dimensional culture Morphology and proliferation of hCCMCs were studied under 3D state. Results: The morphology of the primary hCCMCs was heterogeneous. After gradient velocity sedimentation, the morphology of hCCMCs became homogeneous. Positive expression of α-SMA was detected in most of hCCMCs by immunohistochemistry. hCCMCs grew at different levels in three-dimensional culture system.No significant proliferation was found in hCCMCs after 10 days three-dimensional culture(P>0.05), Proliferation rate of them was significantly lower than that of adherent monolayer cells(P<0.01). Conclusion: Three-dimensional culture system could be used to detect the growth and proliferation of hCCMCs under the three-dimensional conditions, and study the interaction of hCCMCs and micro-environment. This research was expected to find a simpler and more controllable alternative to related experiments in vivo.
语种：
中文
第一作者：
第一作者单位：[1]Department of Urology,Tongji Hospital,Huazhong University of Science and Technology,Wuhan,Hubei 430030,China
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推荐引用方式(GB/T 7714)：
Chen R,Liu J,Rao K,等.Establishment of the three-dimensional culture model of human corpus cavernosum smooth muscle cells[J].中国男科学杂志.2010,24(2):
