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Cancer Cell Membrane Vesicle for Multiplex MicroRNA Imaging in Living Cells

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单位： [a]School of Chemistry and Bioengineering, University of Science and Technology Beijing, Beijing, 100083, China [b]Department of Surgery and Department of Gerontology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,430074,China [c]Department of Orthopedic Surgery, Jiangxi Province People's Hospital, Nanchang, Jiangxi, 330006, China [d]College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, China

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Highly efficient cellular transfection and intracellular signal amplification is a prerequisite for low-abundant microRNA (miRNA) imaging and biomedical application. Herein, we report a functional cancer cell membrane (CM) vesicle, Au-P/[email protected] (DSN, double-specific nucleases), which consists of Au nanoparticles modified with three types of fluorescent miRNA detection probes (Au-P) and DSN that simultaneously encapsulate in cancer CM. We find that the Au-P/[email protected] could specifically target the cancer cell and transfect the cell with higher efficiency than Au nanoparticles. The internalized Au-P/[email protected] could further specifically recognize the target miRNA and induce DSN-assisted target recycle signal amplification, leading to multiple miRNA simultaneous detection with high sensitivity. It successfully detects oncogenic miRNAs in MCF-7 cells with high sensitivity and is amenable to monitor the dynamic expression change of oncogenic miRNAs in cancer cells. Our study represents a promising gene delivery vector for cancer diagnosis and potential therapy. © 2019 American Chemical Society.

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出版当年[2019]版：

大类 | 1 区

化学

小类 | 1 区分析化学

最新[2025]版：

大类 | 1 区

化学

小类 | 1 区分析化学

第一作者：

第一作者单位： [a]School of Chemistry and Bioengineering, University of Science and Technology Beijing, Beijing, 100083, China

通讯作者：

通讯机构： [a]School of Chemistry and Bioengineering, University of Science and Technology Beijing, Beijing, 100083, China [b]Department of Surgery and Department of Gerontology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,430074,China [d]College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, China

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