We observed that BMSCs (bone marrow stromal cells) from myeloma patients (myeloma BMSCs) were significantly stiffer than control BMSCs using a cytocompression device. The stiffness of myeloma BMSCs and control BMSCs was further increased upon priming by myeloma cells. Additionally, myeloma cells became stiffer when primed by myeloma BMSCs. The focal adhesion kinase activity of myeloma cells was increased when cells were on stiffer collagen gels and on myeloma BMSCs. This change in myeloma stiffness is associated with increased colony formation of myeloma cells and FAK activation when co-cultured with stiffer myeloma BMSCs or stiffer collagen. Additionally, stem cells of RPMI8226 cells became stiffer after priming by myeloma BMSCs, with concomitant increases of stem cell colony formation. These results suggest the presence of a mechanotransduction loop between myeloma cells and myeloma BMSCs to increase the stiffness of both types of cells via FAK activation. The increase of stiffness may in turn support the growth of myeloma cells and myeloma stem cells. (C) 2009 Elsevier Ltd. All rights reserved.
第一作者单位：[1]Methodist Hosp, Dept Pathol, Houston, TX 77030 USA[2]Methodist Hosp, Res Inst, Houston, TX 77030 USA[3]Huangzhong Univ Sci & Technol, Tongji Med Coll, Dept Surg, Tongji Hosp, Wuhan, Peoples R China
通讯作者：
通讯机构：[1]Methodist Hosp, Dept Pathol, Houston, TX 77030 USA[2]Methodist Hosp, Res Inst, Houston, TX 77030 USA[6]Cornell Univ, Weill Med Coll, Dept Pathol, New York, NY 10021 USA[*1]Methodist Hosp, Dept Pathol, 6565 Fannin,MS205, Houston, TX 77030 USA
推荐引用方式(GB/T 7714)：
Feng Yongdong,Ofek Gidon,Choi Dong Soon,et al.Unique biomechanical interactions between myeloma cells and bone marrow stroma cells[J].PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY.2010,103(1):148-156.doi:10.1016/j.pbiomolbio.2009.10.004.
APA：
Feng, Yongdong,Ofek, Gidon,Choi, Dong Soon,Wen, Jianguo,Hu, Jerry...&Chang, Chung-Che.(2010).Unique biomechanical interactions between myeloma cells and bone marrow stroma cells.PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY,103,(1)
MLA：
Feng, Yongdong,et al."Unique biomechanical interactions between myeloma cells and bone marrow stroma cells".PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY 103..1(2010):148-156
：