Low-intensity red laser light can promote cell proliferation through a wild network of signal pathways. Mammalian target of rapamycin (mTOR) pathway plays multiple roles in cell growth, proliferation, and survival. The aim of this paper is to investigate the activation of mTOR after rat bone marrow mesenchymal stem cells (MSCs) were irradiated by red light from a light-emitting diode (LED). The cells were seeded in 35mm tissue-culture dishes at a density of 8x10(4) cells/dish, a 96-well ELISA plate at a density of 3x10(3) cells/well, and cultured in Dulbecco's modified Eagle's medium (DMEM). BMSCs were irradiated once by 620nm red light with radiation energies 0.5 J/cm(2), IJ/cm(2), 2J/cm(2). Methyltetra-zolium (MIT) assay indicated that irradiated cells significantly proliferated. This effect was confirmed by higher activity of phosphorylates mTOR. In conclusion, mTOR signal pathway may involve in red light irradiation triggered cell proliferation.
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外文
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第一作者：
第一作者单位：[1]Huazhong Univ Sci & Technol,Dept Orthoped,Tongji Med Coll,Tongji Hosp,Wuhan 430074,Peoples R China
通讯作者：
通讯机构：[1]Huazhong Univ Sci & Technol,Dept Orthoped,Tongji Med Coll,Tongji Hosp,Wuhan 430074,Peoples R China[*1]Huazhong Univ Sci & Technol,Dept Orthoped,Tongji Med Coll,Tongji Hosp,1095 Jiefang Rd,Wuhan 430074,Peoples R China
推荐引用方式(GB/T 7714)：
Peng Fei,Wu Hua,Zheng Yadong.Red Light Irradiation Promotes Mesenchymal Stem Cell Proliferation and Actives Activation of mTOR in Rat[J].6TH WORLD CONGRESS OF BIOMECHANICS (WCB 2010), PTS 1-3.2010,31:1155-1158.doi:10.1007/978-3-642-14515-5_293.
APA：
Peng, Fei,Wu, Hua&Zheng, Yadong.(2010).Red Light Irradiation Promotes Mesenchymal Stem Cell Proliferation and Actives Activation of mTOR in Rat.6TH WORLD CONGRESS OF BIOMECHANICS (WCB 2010), PTS 1-3,31,
MLA：
Peng, Fei,et al."Red Light Irradiation Promotes Mesenchymal Stem Cell Proliferation and Actives Activation of mTOR in Rat".6TH WORLD CONGRESS OF BIOMECHANICS (WCB 2010), PTS 1-3 31.(2010):1155-1158
