详情页 - 聚合机构库演示平台

当前位置：首页 > 详情页

Inhibitory function of Tregs via soluble FGL2 in chronic hepatitis B

110| 认领 | 导出 | 链接全文 |

文献详情

资源类型：

WOS体系：

Pubmed体系：

收录情况： ◇ SCIE

作者：

单位： [1]Huazhong Univ Sci & Technol, Dept & Inst Infect Dis, Tongji Med Coll, Tongji Hosp, Wuhan 430030, Peoples R China

出处：

DOI：

ISSN：

关键词： soluble FGL2 protein regulatory T cells CD8(+) T cells chronic hepatitis B

摘要：

CD4(+)CD25(+)CD127(dim/-) regulatory T cells (Tregs) have been implicated in suppressing T cell immune responses to hepatitis B virus (HBV), but the inhibition mechanism has not being clear yet. This study investigated the effects of soluble FGL2 (sFGL2) secreted by Tregs on immune suppression in chronic HBV-infected patients. We verified that sFGL2 protein and mRNA were highly expressed in Tregs. The separated Tregs by using magnetic beads from peripheral blood mononuclear cells (PBMCs) in 20 patients with chronic hepatitis B were co-cultured with PBMCs at a ratio of 1:3 with anti-CD3 stimulating antibody or FGL2 blocking antibody. The proliferation index of CD8(+)T cells after blocking FGL2 was higher than that in blank group (3.58 +/- 0.18 vs. 3.28 +/- 0.17, P=0.034) in 18 of 20 samples, and lower than that in CD3 stimulation group (3.82 +/- 0.19, P=0.026) in 16 of 20 samples. The IFN-gamma secreted in the mixed culture in the absence of Tregs was higher than that in the culture in the presence of Tregs, but it could be abolished by FGL2 blocking antibody. These results suggest that sFGL2 protein secreted by Tregs suppresses the proliferation and function of CD8(+) T cells in chronic hepatitis B.

基金：

基金编号： 2007CB512900

语种：

被引次数：

WOS：

PubmedID：

中科院(CAS)分区：

出版当年[2011]版：

大类 | 4 区医学

小类 | 4 区生化与分子生物学

最新[2025]版：

无

JCR分区：

出版当年[2010]版：

Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

最新[2023]版：

无

影响因子： 0 最新[2023版] 0 最新五年平均 0.405 出版当年[2010版] 0 出版当年五年平均 0.311 出版前一年[2009版] 0.385 出版后一年[2011版]

第一作者：

第一作者单位： [1]Huazhong Univ Sci & Technol, Dept & Inst Infect Dis, Tongji Med Coll, Tongji Hosp, Wuhan 430030, Peoples R China

通讯作者：

推荐引用方式(GB/T 7714)：

APA：

MLA：

相关文献

[1]Memory CD4(+) T cells are suppressed by CD8(+) regulatory T cells in vitro and in vivo [2]Memory CD4+ T cells are suppressed by CD8+ regulatory T cells in vitro and in vivo. [3]Memory CD4+ T cells are suppressed by CD8+ regulatory T cells in vitro and in vivo [4]Reactivation of BK virus after double umbilical cord blood transplantation in adults correlates with impaired reconstitution of CD4+ and CD8+ T effector memory cells and increase of T regulatory cells [5]Differences in the Induction of Induced Human CD4+ CD25+ FoxP3+ T-Regulatory Cells and CD3+ CD8+ CD28- T-Suppressor Cells Subset Phenotypes In Vitro: Comparison of Phorbol 12-Myristate 13-Acetate/Ionomycin and Phytohemagglutinin Stimulation [6]Pegylated Interferon-α (IFN-α) Enhances the Inhibitory Effect of Natural Killer Cells on Regulatory T Cells via IFN-γ in Chronic Hepatitis B [7]Increased non-MAIT CD161+CD8+ T cells display pathogenic potential in chronic HBV infection [8]Inhibition of Cdk2 promotes the generation of inducible CD8+ T regulatory cells by abrogating binding of the EZH2 methyltransferase on the Foxp3 promoter [9]Combination Therapy With Interferon Plus Interleukin 2 and Hepatitis B Vaccine in Chronic Hepatitis B Patients [10]Strategies to reinvigorate exhausted CD8+ T cells in tumor microenvironment