Rho-associated Kinase (ROCK) has been identified as an important regulator of proliferation and cell cycle progression in a number of cell types. Although its effects on astrocyte proliferation have not been well characterized, ROCK has been reported to play important roles in gap junction formation, morphology, and migration of astrocytes. In the present study, our aim was to investigate the effect of ROCK inhibition by [(+)-(R)-trans-4-(1-aminoethyl)-N-(4-pyridyl) cyclohexanecarboxamide dihydrochloride] (Y27632) on proliferation and DNA synthesis in cultured astrocytes from rat spinal cord and the possible mechanism involved. Western blots showed that treatment of astrocytes with Y27632 increased their expression of cyclin D1, CDK4, and cyclin E, thereby causing cell cycle progression. Furthermore, Y27632-induced astrocyte proliferation was mediated through the extracellular-signal-regulated kinase signaling cascade. These results indicate the importance of ROCK in astrocyte proliferation. (C) 2012 Elsevier Ltd. All rights reserved.
基金：
Natural Science Foundation of China [81000521]; National Basic Research Program [2011CB504403]
第一作者单位：[1]Huazhong Univ Sci & Technol,Tongji Med Coll,Tongji Hosp,Dept Neurol,Wuhan 430030,Peoples R China
通讯作者：
推荐引用方式(GB/T 7714)：
Yu Zhiyuan,Liu Miao,Fu Peicai,et al.ROCK inhibition with Y27632 promotes the proliferation and cell cycle progression of cultured astrocyte from spinal cord[J].NEUROCHEMISTRY INTERNATIONAL.2012,61(7):1114-1120.doi:10.1016/j.neuint.2012.08.003.
APA：
Yu, Zhiyuan,Liu, Miao,Fu, Peicai,Xie, Minjie,Wang, Wei&Luo, Xiang.(2012).ROCK inhibition with Y27632 promotes the proliferation and cell cycle progression of cultured astrocyte from spinal cord.NEUROCHEMISTRY INTERNATIONAL,61,(7)
MLA：
Yu, Zhiyuan,et al."ROCK inhibition with Y27632 promotes the proliferation and cell cycle progression of cultured astrocyte from spinal cord".NEUROCHEMISTRY INTERNATIONAL 61..7(2012):1114-1120
