单位：[1]Department of Urology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China华中科技大学同济医学院附属同济医院外科学系泌尿外科[2]Institute of Urology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China华中科技大学同济医学院附属同济医院外科学系泌尿外科[3]Translational Medicine Center,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China华中科技大学同济医学院附属同济医院转化医学中心党政职能科室科研处[4]Department of Obstetrics and Gynecology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China华中科技大学同济医学院附属同济医院妇产科教研室妇产科学系[5]Department of Urology, Puai Hospital, Wuhan, 430033, China.[6]Department of Cell Death and Cancer Genetics, The Hormel Institute, University of Minnesota, Austin, Minnesota 55912, USA
Purpose: In this study we tried to systematically investigate the tumor suppressing microRNAs in ccRCC. Materials and methods: The MTS cell viability and colony formation assay were used to systematically detect the tumor suppressing ability of down-regulated miRNAs in ccRCC. Then miR-206 expression was detected by RT-qPCR and in situ hybridization in ccRCC cell lines and clinical samples. Oligonucleotides were used to overexpress or down-regulate miR-206. MTS cell viability, EdU cell proliferation, colony formation assay, flow cytometry, Xenograft subcutaneously and orthotopic implantations were done to examine tumor suppressing effects of miR-206 in vitro and in vivo. Luciferase assay was performed to verify the precise target of miR-206. Results: We reviewed and experimentally analyzed the currently available miRNA expression profiles data of ccRCC and identified miR-206 as one of the most critical tumor-suppressing microRNAs in ccRCC. In addition, miR-206 inhibited ccRCC cell proliferation through inducing cell cycle arrest by directly targeting cell cycle related gene CDK4, CDK9 and CCNDI. Conclusions: All these results suggested that miR-206 functioned as a novel cell cycle regulator and tumor suppressor in ccRCC and could be considered as a potential target for ccRCC therapy. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31372562, 81170650, 81270788, 81470935, 81402098, 81402105, 81402087]; National Major Scientific and Technological Special Project for Significant New Drugs Development [2012ZX09303018]; Chenguang Program of Wuhan Science and Technology Bereau [2013072304010833, 2015070404010199]; National High Technology Research and Development Program 863National High Technology Research and Development Program of China [2014AA020607]; Natural Science Foundation of Hubei ProvinceNatural Science Foundation of Hubei Province [2015CFB289, 2012FFB02412, 2013CFB174]
第一作者单位：[1]Department of Urology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China[2]Institute of Urology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Urology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China[6]Department of Cell Death and Cancer Genetics, The Hormel Institute, University of Minnesota, Austin, Minnesota 55912, USA
推荐引用方式(GB/T 7714)：
xiao haibing,xiao wei,cao jing,et al.miR-206 functions as a novel cell cycle regulator and tumor suppressor in clear-cell renal cell carcinoma[J].CANCER LETTERS.2016,374(1):107-116.doi:10.1016/j.canlet.2016.01.032.
APA：
xiao,haibing,xiao,wei,cao,jing,li,heng,guan,wei...&xu,hua.(2016).miR-206 functions as a novel cell cycle regulator and tumor suppressor in clear-cell renal cell carcinoma.CANCER LETTERS,374,(1)
MLA：
xiao,haibing,et al."miR-206 functions as a novel cell cycle regulator and tumor suppressor in clear-cell renal cell carcinoma".CANCER LETTERS 374..1(2016):107-116
医学