Ten new alkaloids (1-10), including two pairs of enantiomeric mixtures (5a,b and 6a,b), and 15 known analogues (11-25) were obtained from the bark of Pausinystalia yohimbe. The structures of 1-25 were established by spectroscopic methods, and the absolute configurations of compounds 1-10 were resolved by X-ray diffraction and ECD data analyses. The in vitro immunosuppressive activities of selected isolates were tested. Compounds 11 and 16 exhibited moderate inhibition with IC50 values of 16.8 and 27.6 mu M against ConA-induced T lymphocyte proliferation and 13.5 and 40.5 mu M against LPS-induced B lymphocyte proliferation, respectively.
基金：
National Natural Science Foundation of China [31770380, 21572073, 31270394, 31500282]; Fundamental Research Funds for the Central Universities [2016YXMS150]
第一作者单位：[1]Huazhong Univ Sci & Technol, Hubei Key Lab Nat Med Chem & Resource Evaluat, Sch Pharm, Tongji Med Coll, Wuhan 430030, Hubei, Peoples R China[2]Wuhan Univ, Zhongnan Hosp, Med Sci Res Ctr, Wuhan 430071, Hubei, Peoples R China
通讯作者：
推荐引用方式(GB/T 7714)：
Liu Ye,Yu Heng-Yi,Xu Hong-Zhe,et al.Alkaloids with Immunosuppressive Activity from the Bark of Pausinystalia yohimbe[J].JOURNAL OF NATURAL PRODUCTS.2018,81(8):1841-1849.doi:10.1021/acs.jnatprod.8b00324.
APA：
Liu, Ye,Yu, Heng-Yi,Xu, Hong-Zhe,Liu, Jun-Jun,Meng, Xiang-Gao...&Ruan, Han-Li.(2018).Alkaloids with Immunosuppressive Activity from the Bark of Pausinystalia yohimbe.JOURNAL OF NATURAL PRODUCTS,81,(8)
MLA：
Liu, Ye,et al."Alkaloids with Immunosuppressive Activity from the Bark of Pausinystalia yohimbe".JOURNAL OF NATURAL PRODUCTS 81..8(2018):1841-1849
