To explore the role of whole-lesion histogram analysis of apparent diffusion coefficient (ADC) for discriminating between T stages of rectal carcinoma by comparison of reduced field-of-view (FOV) and conventional DWI techniques. 102 patients with rectal cancer were enrolled in this retrospective study. All patients received preoperative MR scan at 3T, including reduced and full FOV DWI sequences. Histogram parameters from two DWI methods were calculated and correlated with histological T stage of rectal cancer. The diagnostic performance of individual parameter for differentiating stage pT1-2 and pT3-4 tumors from both DWI techniques was assessed by receiver operating characteristic curve analysis. There were significant differences for the parameters of ADCmean, 50th, 75th, 90th, 95th percentiles, skewness and kurtosis of both DWI sequences in patients with pT1-2 as compared to those with pT3-4 tumors (P < 0.05), in addition to parameters including ADCmin (P =0.015) and 25th percentile (P = 0.006) from rFOV DWI. Correlations were noted between T staging and above histogram parameters from rFOV DWI (r: -0.741-0.682) and fFOV DWI (r: -0.449-0.449), besides parameters of ADCmin (0.370) and 25th percentile (-0.425) from rFOV DWI. The AUCs of 75th and 90th percentiles from rFOV DWI were significantly higher than that from fFOV DWI (P = 0.0410 and P = 0.0208). The whole-lesion histogram analysis based on rFOV DWI was overall more advantageous than the one based on fFOV DWI in differentiating T staging of rectal cancer and the 90th percentile ADC from rFOV DWI was the value with the highest AUC (0.932).