Assessment of trace element levels in general population from the specific area is of importance for nutritional and occupational monitoring. In the current study, baseline blood levels of 12 toxic and/or essential metals and metalloids, including arsenic (As), cadmium (Cd), lead (Pb), mercury (Hg), chromium (Cr), thallium (Tl), manganese (Mn), copper (Cu), Zinc (Zn), calcium (Ca), iron (Fe), and magnesium (Mg), in general populations (n=477) of Wuhan in central China were investigated by using inductively coupled plasma mass spectrometry (ICP-MS). The geometric means for As, Cd, Pb, Hg, Cr, Tl, Mn, and Cu were measured as 2.25, 0.70, 17.84, 1.90, 0.36, <0.05, 12.40, and 783.76g/L, respectively. The geometric means for Zn, Ca, Fe, and Mg were 5.85, 56.66, 488.98, and 39.44mg/L, respectively. We found the men had higher blood As, Pb, Hg, Zn, Fe, and Mg levels but had lower blood Cu and Ca levels than the women (p<0.05). Age-related difference were found for blood Cu, Zn, Ca, Mg, Pb, Mn, As, Cd, and Hg levels (p<0.05). Moreover, many metal concentrations were found correlated, with the strongest correlations between the pairs Fe-Mg (r=0.57), Fe-Zn (r=0.42), As-Hg (r=0.46), Ca-Cu (r=0.34), Pb-Hg (r=0.36), Pb-Cd (r=0.31), Pb-As (r=0.25), and Ca-Fe (r=-0.23). Compared with reports from other countries, most of our results were consistent, except that As Pb, Hg, Mn, and Cu showed different blood levels with European, Korea, or Beijing areas. Our study would be of importance for nutritional, environmental, and/or occupational monitoring of these metals in human.