Background: Putative endothelial progenitor cells (pEPCs) have been confirmed to participate in alleviation of renal fibrosis in several ischaemic diseases. However, their mechanistic effect on renal fibrosis, which is characterized by vascular regression and further rarefaction-related pathology, remains unknown. Methods: To explore the effect and molecular mechanisms by which pEPCs act on unilateral ureteral obstruction (UUO)-induced renal fibrosis, we isolated pEPCs from murine bone marrow. In vivo, pEPCs (2x10(5) cells/day) and pEPC-MVs (microvesicles) were injected into UUO mice via the tail vein. In vitro, pEPCs were co-cultured with renal-derived pericytes. Pericyte-myofibroblast transition was evaluated using the myofibroblast marker alpha-smooth muscle actin (alpha-SMA) and pericyte marker platelet-derived growth factor receptor beta (PDGFR-beta). Results: Exogenous supply of bone marrow-derived pEPCs attenuated renal fibrosis by decreasing pericyte-myofibroblast transition without significant vascular repair in the UUO model. Our results indicated that pEPCs regulated pericytes and their transition into myofibroblasts via pEPC-MVs. Co-culture of pericytes with pEPCs in vitro suggested that pEPCs inhibit transforming growth factor-beta (TGF-beta)-induced pericyte-myofibroblast transition via a paracrine pathway. Conclusionp: EPCs effectively attenuated UUO-induced renal fibrosis by inhibiting pericyte-myofibroblast transition via a paracrine pathway, without promoting vascular repair.
基金：
National Natural Science Foundation of China [81570669, 81770684, 81200521, 81570615, 81770681]; Research Project of Hubei Provincial Department of Education [2014070]; Wuhan health and family planning commission [2016whzqnyxggrc2]
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2018]版：
大类|2 区医学
小类|2 区医学：研究与实验3 区细胞生物学
最新[2025]版：
大类|2 区医学
小类|2 区细胞与组织工程2 区细胞生物学2 区医学：研究与实验
JCR分区：
出版当年[2017]版：
Q1MEDICINE, RESEARCH & EXPERIMENTALQ2CELL BIOLOGY
最新[2024]版：
Q1CELL & TISSUE ENGINEERINGQ1CELL BIOLOGYQ1MEDICINE, RESEARCH & EXPERIMENTAL
第一作者单位：[1]Huazhong Univ Sci & Technol, Tongji Med Coll, Tongji Hosp, Dept Nephrol, 1095 Jiefang Ave, Wuhan 430030, Hubei, Peoples R China
通讯作者：
通讯机构：[1]Huazhong Univ Sci & Technol, Tongji Med Coll, Tongji Hosp, Dept Nephrol, 1095 Jiefang Ave, Wuhan 430030, Hubei, Peoples R China[2]Huazhong Univ Sci & Technol, Tongji Med Coll, Tongji Hosp, Dept Nutr, 1095 Jiefang Ave, Wuhan 430030, Hubei, Peoples R China
推荐引用方式(GB/T 7714)：
Yang Juan,Wang Meng,Zhu Fengming,et al.Putative endothelial progenitor cells do not promote vascular repair but attenuate pericyte-myofibroblast transition in UUO-induced renal fibrosis[J].STEM CELL RESEARCH & THERAPY.2019,10:doi:10.1186/s13287-019-1201-5.
APA：
Yang, Juan,Wang, Meng,Zhu, Fengming,Sun, Jie,Xu, Huzi...&Yao, Ying.(2019).Putative endothelial progenitor cells do not promote vascular repair but attenuate pericyte-myofibroblast transition in UUO-induced renal fibrosis.STEM CELL RESEARCH & THERAPY,10,
MLA：
Yang, Juan,et al."Putative endothelial progenitor cells do not promote vascular repair but attenuate pericyte-myofibroblast transition in UUO-induced renal fibrosis".STEM CELL RESEARCH & THERAPY 10.(2019)
医学