单位：[1]Department of Cardiology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China华中科技大学同济医学院附属同济医院内科学系心血管内科
The aim of this paper is to study the effect of bradykinin(BK)on bradykinin-B2 receptor as well as the possible involved signal transduction pathways in cultured rat aortic vascular smooth muscle cells(VSMCs).Rat aortic VSMCs were cultured.Cells after 4-6 passages were used in the experiment.VSMCs were incubated with BK,BK+B2 receptor inhibitor(HOE-140),BK+MEK inhibitor(PD98059),BK+mitogen-activated protein kinase(MAPK)inhibitor(apigenin),BK+phosphoinositide 3-kinase(PI3K)inhibitor(LY294002),and BK+Akt inhibitor to evaluate the expression of B2 receptor and phosphorylation of signaling molecules MAPK,Akt,and PI3K by Western blot.(1)BK markedly up-regulated the expression of B2 receptor in VSMC.(2)Apigenin,PD98059,Akt inhibitor,and LY294002 inhibited upregulation of B2 receptor induced by BK.(3)Signal transduction pathways of MAPK and PI3K were involved in the up-regulation of B2 receptor by BK mediation.Results suggest that bradykinin can up-regulate the expression of B2 receptor in VSMCs.
语种：
外文
第一作者：
第一作者单位：[1]Department of Cardiology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China
通讯作者：
推荐引用方式(GB/T 7714)：
Yan W,Feng M,Wang P.-H,et al.Effect of bradykinin on bradykinin-B2 receptor in rat aortic vascular smooth muscle cells and the involved signal transduction pathways[J].FRONTIERS OF MEDICINE IN CHINA.2010,4(2):225-228.doi:10.1007/s11684-010-0003-z.
APA：
Yan, W,Feng, M,Wang, P.-H&Wang, D.-W.(2010).Effect of bradykinin on bradykinin-B2 receptor in rat aortic vascular smooth muscle cells and the involved signal transduction pathways.FRONTIERS OF MEDICINE IN CHINA,4,(2)
MLA：
Yan, W,et al."Effect of bradykinin on bradykinin-B2 receptor in rat aortic vascular smooth muscle cells and the involved signal transduction pathways".FRONTIERS OF MEDICINE IN CHINA 4..2(2010):225-228
