单位:[1]School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, People's Republic of China.[2]Department of Oncology, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, People's Republic of China.肿瘤科华中科技大学同济医学院附属同济医院
Toehold-mediated DNA strand displacement is the foundation of dynamic DNA nanotechnology, encompassing a wide range of tools with diverse functions, dynamics, and thermodynamic properties. However, a majority of these tools are limited to unidirectional reactions driven by thermodynamics. In response to the growing field of dissipative DNA nanotechnology, we present an approach: DNAzyme-based dissipative DNA strand displacement (D-DSD), which combines the principles of dynamic DNA nanotechnology and dissipative DNA nanotechnology. D-DSD introduces circular and dissipative characteristics, distinguishing it from the unidirectional reactions observed in conventional strand displacement. We investigated the reaction mechanism of D-DSD and devised temporal control elements. By substituting temporal components, we designed two distinct temporal AND gates using fewer than 10 strands, eliminating the need for complex network designs. In contrast to previous temporal logic gates, our temporal storage is not through dynamics control or cross-inhibition but through autoregressive storage, a more modular and scalable approach to memory storage. D-DSD preserves the fundamental structure of toehold-mediated strand displacement, while offering enhanced simplicity and versatility.
基金:
This work was financially supported by the National Natural
Science Foundation of China (No. 82172372) and the
Opening Research Fund of State Key Laboratory of Digital
Medical Engineering (No. 2023-M04).
第一作者单位:[1]School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, People's Republic of China.[2]Department of Oncology, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, People's Republic of China.
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通讯作者:
推荐引用方式(GB/T 7714):
Hu Minghao,Li Xiaolong,Wu Jia-Ni,et al.DNAzyme-Based Dissipative DNA Strand Displacement for Constructing Temporal Logic Gates[J].ACS Nano.2024,18(3):2184-2194.doi:10.1021/acsnano.3c09506.
APA:
Hu Minghao,Li Xiaolong,Wu Jia-Ni,Yang Mengyao&Wu Tongbo.(2024).DNAzyme-Based Dissipative DNA Strand Displacement for Constructing Temporal Logic Gates.ACS Nano,18,(3)
MLA:
Hu Minghao,et al."DNAzyme-Based Dissipative DNA Strand Displacement for Constructing Temporal Logic Gates".ACS Nano 18..3(2024):2184-2194