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CAPA-IVM improves the cytoplasmic quality of in vitro-matured oocytes from unstimulated mice

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作者：
Gong Xueqi[1] * ; Shen Lin[1] ; Zhang Hanwang[1] ; Ai Jihui[1] ; Gilchrist Robert B[2] ; Zhao Yiqing[1] ;
单位： [1]Reproductive Medicine Center,Department of Gynecology and Obstetrics,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,1095 Jiefang Avenue,Wuhan,430030,China [2]Discipline of Women’s Health, School of Clinical Medicine, University of New South Wales Sydney, NSW, 2052, Australia
出处：
DOI： 10.1016/j.theriogenology.2023.09.004
ISSN：

关键词： Capacitation-IVM (CAPA-IVM) Ovarian tissue oocytes (OTOs) Reactive oxygen species (ROS) Mitochondria Cytoplasmic quality

摘要：
Ovarian tissue oocyte (OTO) in vitro maturation (IVM) is a strategy to improve fertility preservation efficiency. Here, the effects of capacitation IVM (CAPA-IVM) on OTO function were investigated. Immature cumulus-oocyte complexes (COCs) from unstimulated 28-day-old mouse ovaries (mimicking OTOs) underwent CAPA-IVM, standard IVM (S-IVM) or in vivo maturation following ovarian stimulation (OS; positive control), and oocyte meiotic maturation and cytoplasmic quality were assessed. CAPA-IVM resulted in improved oocyte meiotic maturation (P < 0.05) and cumulus expansion (P < 0.0001) compared to S-IVM, with expansion comparable to the OS group. MII OTO ROS was lower after CAPA-IVM than S-IVM (P < 0.0001) but not as low as in the OS group (P = 0.036). CAPA-IVM resulted in a better oocyte mitochondrial distribution than S-IVM (P < 0.05) and was similar to the OS group (P > 0.05). Mitochondrial membrane potential in MII OTOs was higher after CAPA-IVM than S-IVM and OS (P < 0.0001). Compared with S-IVM, CAPA-IVM resulted in lower rates of spindle/chromosome configuration and cortical granule distribution abnormalities (P < 0.05), which were similar to OS levels (P > 0.05). MII OTO intracellular Ca2+ levels were similar in the CAPA-IVM and OS groups (P > 0.05), while S-IVM decreased intracellular Ca2+ (P < 0.05). CAPA-IVM and S-IVM decreased mitochondrial Ca2+ levels (P < 0.05). CAPA-IVM increased expression of antioxidant genes (Sod2 and Sirt1) and Egfr (P < 0.05) but not apoptotic genes (Bcl2, Bax and Bcl2/Bax; P > 0.05). CAPA-IVM increased the OTO maturation rate and quality of oocytes from unstimulated mice to the extent that many features of oocyte cytoplasmic quality were comparable to superovulated in vivo matured oocytes.Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.

基金：
This work was supported by the Clinical Medical Research Special Fund of the Chinese Medical Association-Reproductive Medicine Research and Development Program for Young Physicians (18010080737), the Basic Research Project for Young and Middle-Aged Physicians from the Beijing Health Promotion Association (BJHPA- 2022-SHZHYXZHQNYJ-JICH-002) and Scientific Research Starting Foundation for the Returned Overseas Scholars (2020HGRY004) from Tongji Hospital.
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外文
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出版当年[2022]版：
大类 | 2 区 农林科学
小类 | 1 区 兽医学 2 区 生殖生物学
最新[2025]版：
大类 | 2 区 农林科学
小类 | 1 区 兽医学 3 区 生殖生物学
JCR分区：
出版当年[2021]版：
Q1 VETERINARY SCIENCES Q3 REPRODUCTIVE BIOLOGY
最新[2024]版：
Q1 VETERINARY SCIENCES Q2 REPRODUCTIVE BIOLOGY

影响因子： 最新[2024版] 最新五年平均 出版当年[2021版] 出版当年五年平均 出版前一年[2020版] 出版后一年[2022版]

第一作者：
第一作者单位： [1]Reproductive Medicine Center,Department of Gynecology and Obstetrics,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,1095 Jiefang Avenue,Wuhan,430030,China
通讯作者：
推荐引用方式(GB/T 7714)：
Gong Xueqi,Shen Lin,Zhang Hanwang,et al.CAPA-IVM improves the cytoplasmic quality of in vitro-matured oocytes from unstimulated mice[J].THERIOGENOLOGY.2023,212:117-128.doi:10.1016/j.theriogenology.2023.09.004.
APA：
Gong Xueqi,Shen Lin,Zhang Hanwang,Ai Jihui,Gilchrist Robert B&Zhao Yiqing.(2023).CAPA-IVM improves the cytoplasmic quality of in vitro-matured oocytes from unstimulated mice.THERIOGENOLOGY,212,
MLA：
Gong Xueqi,et al."CAPA-IVM improves the cytoplasmic quality of in vitro-matured oocytes from unstimulated mice".THERIOGENOLOGY 212.(2023):117-128

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