单位：[1]Department of Laboratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.检验科华中科技大学同济医学院附属同济医院[2]College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.[3]Hubei Key Laboratory of Agricultural Bioinformatics, College of Informatics, Huazhong Agricultural University, Wuhan 430070, China.[4]Key Laboratory for Deep Processing of Major Grain and Oil of Ministry of Education, Wuhan Polytechnic University, Wuhan 430023, China.[5]Department of Emergency, Wuhan Municipal Fourth Hospital Affiliated Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430034, China.[6]Jiangsu Provincial Key Laboratory of Critical Care Medicine, Department of Critical Care Medicine, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 210009, China.[7]Department of Critical Care Medicine, Union Jiangbei Hospital, Huazhong University of Science and Technology, Wuhan 430100, China.
Obesity represents a major health challenge because it substantially increases the risk of metabolic diseases. Capsaicin, the major active ingredient of Capsicum spp., has been reported to possess anti-obesity activity. Hereon, the effect of capsaicin on glucose uptake and consumption in hepatocytes was extensively studied. Capsaicin was shown to accelerate the glucose uptake/consumption and the ATP production of hepatocytes. The elevation of intracellular Ca2+ was thought to be a potential mechanism. By transcriptome analysis, 78, 146 and 507 differentially expressed genes (DEGs) were identified between capsaicin and the control group for 4 h, 12 h and 24 h treatments. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that most of the DEGs were involved in canonical pathways, like MAPK and PI3K-AKT signaling pathways. Clustering analysis showed that many DEGs were associated with glucose and amino acid metabolism. The variation trend in genes related to glucose and amino acid metabolism (like CTH, VEGFA, PCK2 and IGFBP3) in the quantitative PCR (q-PCR) assay was consistent with the transcriptome data. These results demonstrated that capsaicin efficiently accelerated the glucose uptake and consumption of hepatocytes.
基金：
National Natural Science Foundation of China (Grant
No. 31972019), Fundamental Research Funds for the Central Universities (Grant No. 2662023PPY003).
第一作者单位：[1]Department of Laboratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
通讯作者：
通讯机构：[6]Jiangsu Provincial Key Laboratory of Critical Care Medicine, Department of Critical Care Medicine, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 210009, China.[7]Department of Critical Care Medicine, Union Jiangbei Hospital, Huazhong University of Science and Technology, Wuhan 430100, China.
推荐引用方式(GB/T 7714)：
Zeng Haolong,Shi Nian,Peng Wenlei,et al.Effects of Capsaicin on Glucose Uptake and Consumption in Hepatocytes[J].MOLECULES.2023,28(13):doi:10.3390/molecules28135258.
APA：
Zeng Haolong,Shi Nian,Peng Wenlei,Yang Qing,Ren Jingnan...&Guo Jun.(2023).Effects of Capsaicin on Glucose Uptake and Consumption in Hepatocytes.MOLECULES,28,(13)
MLA：
Zeng Haolong,et al."Effects of Capsaicin on Glucose Uptake and Consumption in Hepatocytes".MOLECULES 28..13(2023)
化学