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Single-Cell RNA sequencing highlights the regulatory role of T cell marker genes Ctla4, Ccl5 and Tcf7 in corneal allograft rejection of mouse model

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单位： [1]Obstetrics and Gynecology Department, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, PR China [2]Aier Eye Hospital of Wuhan University, Wuhan 430070, PR China [3]School of Optometry, Hubei University of Science and Technology, Xianning 437100, PR China

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关键词： Corneal allograft Immune rejection Single -cell RNA sequencing CD4+T cells Tcf7 Ctla4 Ccl5

摘要：

Background: A mouse corneal allograft model was induced and single-cell RNA sequencing (scRNA-seq) data of corneal tissues and T cells were analyzed to reveal a T cell-mediated mechanism for corneal allograft rejection in mice. Methods: Corneal tissue samples from a mouse model of corneal allograft were collected for scRNA-seq analysis, followed by quality control, dimensionality reduction, cluster analysis and enrichment analysis. A large number of highly variable genes were identified in mice with corneal allograft. Significant difference existed in immune T cells, especially in CD4 + T cells. Results: It was found that T cell marker genes Ctla4, Ccl5, Tcf7, Lgals1, and Itgb1 may play key roles in the corneal allograft rejection. Mice with allograft rejection showed a significant increase in the proportion of CD4 + T cells in the corneal tissues. Besides, Ccl5 and Tcf7 expression was increased in mice with allograft rejection and positively linked to the proportion of CD4 + T cells. Whereas, Ctla4 expression was downregulated and negatively associated with the proportion of CD4 + T cells. Conclusion: Collectively, Ctla4, Ccl5 and Tcf7 may participate in the rejection of corneal allograft in mice by affecting CD4 + T cell activation.

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出版当年[2022]版：

大类 | 2 区医学

小类 | 2 区免疫学 2 区药学

最新[2025]版：

大类 | 2 区医学

小类 | 2 区药学 3 区免疫学

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出版当年[2021]版：

Q1 PHARMACOLOGY & PHARMACY Q2 IMMUNOLOGY

最新[2023]版：

Q1 PHARMACOLOGY & PHARMACY Q2 IMMUNOLOGY

影响因子： 4.8 最新[2023版] 5 最新五年平均 5.714 出版当年[2021版] 5.597 出版当年五年平均 4.932 出版前一年[2020版] 5.6 出版后一年[2022版]

第一作者：

第一作者单位： [1]Obstetrics and Gynecology Department, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, PR China

通讯作者：

通讯机构： [2]Aier Eye Hospital of Wuhan University, Wuhan 430070, PR China [3]School of Optometry, Hubei University of Science and Technology, Xianning 437100, PR China [*1]Aier Eye Hospital of Wuhan University, No. 481, Zhongshan Road, Wuchang District, Wuhan 430070, Hubei Province, PR China.

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