Objective. To study the effect of circ_0000512 (circRPPH1_025) on the tumorigensis and development of glioblastoma and its molecular mechanism. Methods. The expression levels of circ_0000512 in normal astrocytes (NHA) and human glioblastoma cell lines (U87, U251, and A172) and the expression levels of circ_0000512 and linear RNA RPPH1 in U87 cells after RNase R treatment were detected by qRT-PCR. The effects of circ_0000512 knockdown or overexpression on the proliferation, migration, invasion, and epithelial-mesenchymal transition of U87 cells were detected by CCK-8 assay, cell colony formation assay, transwell invasion assay, wound healing assay, and western blot. Results. The expression of circ_0000512 was upregulated in glioblastoma cells, and the overexpression of circ_0000512 was beneficial to the proliferation, migration, invasion, and epithelial-mesenchymal transition of U87 cells, while knockdown of circ_0000512 showed the opposite results. Conclusion. circ_0000512 can be used as a potential target for early diagnosis and targeted therapy of glioblastoma multiforme.
基金：
[2021RC006]
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2021]版：
大类|4 区医学
小类|4 区生物工程与应用微生物4 区遗传学4 区医学：研究与实验4 区病理学
最新[2025]版：
无
JCR分区：
出版当年[2020]版：
Q2BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ2PATHOLOGYQ2GENETICS & HEREDITYQ3MEDICINE, RESEARCH & EXPERIMENTAL
第一作者单位：[1]Shanxi Med Univ, Hosp 3, Tongji Shanxi Hosp, Shanxi Acad Med Sci,Dept Neurosurg,Shanxi Bethune, Taiyuan 030032, Peoples R China[2]Huazhong Univ Sci & Technol,Tongji Hosp,Tongji Med Coll,Dept Neurosurg,Wuhan 430030,Peoples R China
通讯作者：
通讯机构：[1]Shanxi Med Univ, Hosp 3, Tongji Shanxi Hosp, Shanxi Acad Med Sci,Dept Neurosurg,Shanxi Bethune, Taiyuan 030032, Peoples R China[2]Huazhong Univ Sci & Technol,Tongji Hosp,Tongji Med Coll,Dept Neurosurg,Wuhan 430030,Peoples R China
推荐引用方式(GB/T 7714)：
Xue Lixiong,Chen Huahui,Wang Xiaolong,et al.circRPPH1_025 Overexpression Promotes Migration and Invasion of Glioblastoma Multiforme[J].DISEASE MARKERS.2022,2022:doi:10.1155/2022/4764028.
APA：
Xue, Lixiong,Chen, Huahui,Wang, Xiaolong,Han, Li,Liu, Yifan&Ding, Xinmin.(2022).circRPPH1_025 Overexpression Promotes Migration and Invasion of Glioblastoma Multiforme.DISEASE MARKERS,2022,
MLA：
Xue, Lixiong,et al."circRPPH1_025 Overexpression Promotes Migration and Invasion of Glioblastoma Multiforme".DISEASE MARKERS 2022.(2022)
